Requisition Requirements

All samples submitted to the lab must be submitted by authorized personnel and accompanied by a completed UAMS Cytogenetics Laboratory Chromosome Analysis Request Form and must include:

Patient name and address
Accession number
Date of birth
Sex of patient
Name and department of submitting physician and lab
Clinical information (primary diagnosis, transplant information, etc.)
Source of specimen
Date and time specimen collected
Type of tests requested

Sample Requirements

Specimens should be collected under sterile conditions. The primary specimen container (innermost container holding the specimen) must be labeled with at least two patient-specimen identifiers.  All specimens must be properly packaged and labeled to indicate the general nature of the materials transported.  Infectious material must be packaged and shipped in accordance with applicable federal, state, and local regulations.  Ensure that the container is securely packaged to prevent temperature extremes or leakage during transport.

Specimens requiring an anticoagulant must be submitted in sodium heparin. If a Vacutainer is used, gently invert the tube five to ten times; do not shake. Lithium heparin is contraindicated.

If UAMS Cytogenetics transport medium is recommended but not available, other sterile tissue culture media may be substituted.  UAMS Cytogenetic Transport Media contains 6cc of sterile supplemented RPMI media and 12µl sodium heparin (1000 U.S.P.)

Transport and Delivery to Lab

Deliver specimens the day of collection at room temperature accompanied by a completed UAMS Cytogenetics Laboratory Chromosome Analysis Request form. Specimens collected within UAMS should be delivered to the UAMS Clinical Laboratory; specimens collected outside the institution should be delivered directly to the Cytogenetics Laboratory.
EXCEPTION: Pleural effusion specimens optimally should be delivered within one hour of collection.

Specimens should be transported at room temperature. Do not freeze or send samples on ice.

Specimens collected after hours should be kept at room temperature and sent to the UAMS Clinical Laboratory.

EXCEPTION: Solid tissue specimens, if not received the same day as collection, should be refrigerated to inhibit potential bacterial growth.

Sub-optimal or Unacceptable Specimens

Blood and Bone Marrow

1. Clotted specimen
2. Collected in inappropriate anticoagulant
3. Received more than 48 hours after collection
4. Inappropriate or leaky container
5. Inadequate volume (QNS)
6. Unlabeled or mislabeled specimen

Solid Tissue and Tumor

1. Collected under non-sterile conditions
2. Transported in non-sterile, leaky, or dry container
3. Inadequate size sample
4. Received more than 24 hours after collection if not refrigerated,
or 48 hours after collection if refrigerated
5. Unlabeled or mislabeled specimen